Anti-inflammatory and anti-arthritic effects of compounds from Buddleja coriacea

Abstract

Buddleja coriacea (B. coriacea) commonly known as ‘Kiswara’ is used as infusions for stomach and joint pain. This study aims to evaluate the in vitro and in vivo anti-inflammatory and anti-arthritic activity was evaluated by measuring inflammatory parameters (TNF-α, C-reactive protein and Fibrinogen) in murine models of the aqueous extract and isolated compounds of B. coriacea. A bio-guided phytochemical analysis based on NMR/MS was performed identifying three (1-3) compounds of the aqueous extract. All compounds inhibited the production of TNF-α in RAW 264.7 cell line, with IC₅₀ of 13.44 (1), 1.13 (2) and 0.57 μM (3), respectively. In addition, compounds 2 and 3 decreased the levels of TNF-α, C-reactive protein and fibrinogen at a concentration of 5 mg/kg in murine models. Our research shows that the compounds isolated from B. coriacea have anti-inflammatory and anti-arthritic properties, providing scientific evidence for the traditional use of this plant species.

Graphical Abstract

Keywords:

• Loganiaceae
• Buddleja coriacea
• anti-inflammatory
• anti-arthritic
• TNF-α

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This work was supported by the Fundación de la Universidad Autónoma de Madrid (FUAM).