Systematic characterisation of the effective components of five Callicarpa species with UPLC-Q-TOF-MS and evaluation of their anti-hyperuricaemic activity

Abstract

Callicarpa kwangtungensis (C. Kw), C. macrophylla (C. Ma), C. nudiflora (C. Nu), C. formosana (C. Fo), and C. kochiana (C. Ko) were medicinal plant resource in China. In this study, the UPLC/Q-TOF-MS analysis was performed and 151 compounds were identified. PCA analysis metabolic profiles of C. Nu, C. Ko and C. Kw leaves differ significantly from the other two Callicarpa species, while C. Fo and C. Ma share similar chemical constituents. OPLS-DA highlight with an S-plot indicated that there are 14 robust known chemical markers enabling the differentiation between these five Callicarpa plants. C. Ma, C. Nu, and C. Fo leaves extracts treatment effectively reversed the body weight loss, uric acid and creatinine content, hepatic XOD activity, kidney, liver, and ankle tissues injury and inflammation induced by potassium oxonate in hyperuricemia mice. While Ko and C. Kw leaves extracts treatment showed less improvement in hyperuricemia mice.

Graphical Abstract

Keywords:

• Callicarpa
• hyperuricaemia
• OPLS-DA
• PCA
• UPLC/Q-TOF-MS

Disclosure statement

The authors declare no competing financial interest.

Additional information

Funding

This study was supported by the Key Research & Development Plan of Hainan Province (No. ZDYF2018228) and the Science Research Fund of Fairy lake Botanical Garden (2019). Urban Administration and Law Enforcement Bureau of Shenzhen Municipality Research Program (202206), Department of science and technology of Qinghai Province, Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibetan Plateau in Qinghai Province (2020-ZJ-Y40).