Two new phenolic allopyranosides and their analogues from the stems of Viburnum luzonicum Rolfe guided by LC-MS

Abstract

Two new phenolic allopyranosides, named viburluzosides A and B (1, 2), together with eight known phenolic glycosides (3 − 10) were discovered from the stems of Viburnum luzonicum Rolfe under the guidance of LC-MS analyses coupled with bioactivity evaluation. They were purified through various chromatography methods and identified by extensive spectroscopic analyses (¹H and ¹³C NMR, HSQC, HMBC, and HRESIMS) and chemical methods. The in vitro evaluation on α-glucosidase and aldose reductase (AR) inhibitory activities of isolated compounds were conducted. Compounds 1 − 4 and 6 − 9 exhibited α-glucosidase inhibitory activities with IC₅₀ values of 5.35 − 21.34 μM and AR inhibitory activities with IC₅₀ values of 6.21 − 40.06 μM. Moreover, the inhibitory kinetics analyses of compounds 1 and 2 were also performed.

Graphical Abstract

Keywords:

• Viburnum luzonicum Rolfe
• LC-MS guidance
• chemical constituents
• α-glucosidase inhibition
• aldose reductase inhibition

Acknowledgements

We are grateful to the Analytical Detective Center in Yangzhou University for recording spectroscopic spectra.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Open Foundation of Green Pesticides and Biotechnology Key Laboratory of Guizhou University of Ministry of Education (2021GDGP0103/2021GDGP0104).