Isocratic elution based analytical method for quantification of phytoconstituents in Hedychium spicatum and evaluation of their α-glucosidase inhibitory activity

Abstract

An efficient, fast, selective and simple isocratic elution method has been established using ultra performance liquid chromatography coupled to photodiode array detection and electrospray ionisation mass spectrometry (UPLC-PDA-ESI-MS) techniques for the quantification of isolated molecules from Hedychium spicatum rhizomes and assess their α-glucosidase inhibitory activity. Developed analytical method was validated as per the regulatory guidelines such as linearity, selectivity, the limits of detection (LOD) and quantification (LOQ), repeatability and accuracy. Highest concentration of 7-hydroxy hedychenone (4) (76.7 mg/g of dried plant material) was detected in rhizomes. Present results demonstrated parent extract (ethanolic) had the greatest inhibitory effect on α-glucosidase with IC₅₀ values of 4.0 µg/mL. Developed method can be used as a new analytical approach for quality assessment of H. spicatum based herbal formulations and other products. Furthermore, current findings showed that the extract, fraction and isolated metabolites may be a good natural antidiabetic.

Graphical Abstract

Keywords:

• Method development
• quantification
• UPLC-PDA-ESI-MS
• α-glucosidase activity

IHBT Publication number:

• 4946

Acknowledgements

The authors gratefully acknowledge The Director, CSIR-IHBT Palampur (H.P.), India, for continuous encouragement and providing the necessary facilities during the course of study.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the CSIR, New Delhi [grant number MLP-0142] and [grant number HCP-0007]. Authors are also grateful to CSIR, New Delhi for financial support of these projects (HCP-0007 and MLP-0142) under which the work was completed.