Effect of salicylic acid elicitor on antioxidant potential and chemical composition of in vitro raised plants of Berberis asiatica Roxb. ex DC.

Abstract

The present study for the first time investigated the effects of Salicylic acid (SA) (50, 100 and 200 mg/L) on in vitro growth and antioxidant capacity of Berberis asiatica. SA significantly enhanced in vitro shoot multiplication at 100 mg/L concentration. The result showed highest significant increase in antioxidant activity by 50 mg/L SA elicited plantlets in terms of DPPH (2,2-Di-phenyl-1-picryl-hydrazyl) free radical scavenging activity (IC50 32.42 ± 0.51), Ferric Reducing Antioxidant Activity (292.73 ± 2.09 mg AAE/g dw) and Metal Chelating Activity (IC50 13.18 ± 0.52). The Gas Chromatography–Mass Spectrometry (GC–MS) profiling revealed presence of 46, 38, 34 and 29 phytochemicals in control, SA1, SA2 and SA3 methanolic extracts respectively. Among the identified compounds, eight compounds, 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl; 1,3,4,5-tetrahydroxy-cyclohexanecarboxylic acid; 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (and Phytol); hexadecanoic acid, methyl ester; 9,12-Octadecadienoic acid (Z,Z)-,methyl ester; di-n-octyl phthalate; stigmast-5-en-3-ol,(3.beta.)- or ß- sitosterol; and squalene were previously known for antioxidant potential.

Graphical Abstract

Keywords:

• Berberis asiatica
• in vitro propagation
• elicitor
• salicylic acid
• antioxidant assay
• GC-MS

Acknowledgement

The authors are thankful to the Head, Department of Botany, Kumaun University, Nainital, India for providing lab facilities.

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

The author(s) reported there is no funding associated with the work featured in this article.