Three new proaporphine alkaloids from Cissampelos capensis L.f. and their cytotoxic evaluation

Abstract

Three new proaporphine alkaloids, cissamaline (4), cissamanine (5), and cissamdine (6), along with two known compounds (2 and 3) were isolated from total tertiary alakloidal extracts of the leaves and stems of Cissampelos capensis. The new alkaloids were elucidated through comprehensive spectroscopic analysis, including 1 D, 2 D NMR and High-resolution LCMS. The in vitro MTT cytotoxicity was evaluated on Caco-2 cell lines, where all the isolated compounds exhibited cytotoxic effects at concentrations above 250 µM.

Graphical Abstract

Keywords:

• Cissampelos capensis
• proaporphine alkaloids
• cytotoxicity

Acknowledgments

The authors acknowledge their biochemistry collaborators, Prof M van de Venter and Ms S Reddy, plant collection by Mr J Block, and the Herbarium at Nelson Mandela University for authentication of plant species.

Disclosure statement

No potential conflicts of interest were reported by the authors.

Additional information

Funding

This work was funded by the Nelson Mandela University Research Development and student support by the National Research Fund (NRF) of South Africa Scares Skills Scholarship [grant number 108391].