Antioxidant isolation and characterization from the plant Tradescantia spathacea Sw. of the Commelinaceae family

Abstract

A novel bioactive flavan glycoside was isolated by solvent extraction method with the help of Soxhlet apparatus from the methanolic extract of Tradescantia spathacea Sw. Flavan glycoside having molecular formula C₂₀H₂₂O₁₀, melting point 175-178⁰C, molecular weight by ESI-MS m/z (M + H]⁺ 423, optical rotation was[α]²¹_D-45.1(c 0.20 methanol). Its structure was determined (–)-epicatechin 7-O-alpha-L-arabinopyranoside. Various color reactions, chemical degradation (like acid hydrolysis, permethylation, and enzymatic hydrolysis), UV-Visible spectrophotometry, Fourier transforms infrared spectroscopy, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectroscopy were used to establish the structure of compound (–)-(–)-epicatechin 7-O-alpha-L-arabinopyranoside.. A flavan glycoside was also tested with a DPPH assay method for antioxidant activity by using Ascorbic acid as standard. DPPH radical scavenging test data demonstrate that a flavan glycoside possesses potent antioxidant activity so this flavan glycoside can be utilized as a potent antioxidant agent.

Graphical Abstract

Keywords:

• Tradescantia spathacea Sw
• Commelinaceae
• flavan glycoside NMR
• ESI-Mass spectrometry
• FT-IR

Acknowledgment

I would like to thank the CDRI and SAIF Lucknow for recording different spectrum data, as well as the head of the Chemistry Department of Dr. H. S. Gour (Central University) Vishwavidyalaya, Sagar for provided that the required research Lab amenities. Kesar Singh, the writer, is grateful to the University Grant Commission-Non NET scholarship for economic assistance.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.