Dereplication of polar extracts from Croton antisyphiliticus Mart. roots and its anti-inflammatory and antinociceptive potential

Abstract

Croton antisyphiliticus Mart. is a plant popularly used in folk medicine by traditional communities from Brazilian savannah to treat general inflammation. According to ethnopharmacological data, this specie can be considered a source of biologically active molecules for the development of new drugs. Thereby, this study reports the results of the dereplication approach of C. antisyphiliticus roots extracts and the in vivo evaluation of its potential antinociceptive and anti-inflammatory in albino Swiss mice. Based on HPLC coupled to Q-Exactive Orbitrap Mass Spectrometer and using GNPS, a total of thirteen polyphenolic compounds were noticed, including four compounds that have been reported for the first time in the genus Croton. Ethanolic and aqueous roots extracts demonstrated a dose-dependent inhibition for the number of writes, reduced pain induced by formalin and hyperalgesia induced by carrageenan. These extracts also reduced paw edema, cell migration, and myeloperoxidase activity, with effects similar to indomethacin and dexamethasone drugs.

Graphical Abstract

Keywords:

• Croton genus
• endogenous mediators
• feature based molecular networking
• myeloperoxidase
• phenolic compounds

SUBJECT CLASSIFICATION CODES:

• Physical sciences
• chemistry
• natural products

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

We sincerely and gratefully thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico for financial support of this project n° 422513/2016-4.