Antihypertensive effect of patulitrin and other constituents from Tagetes patula L. (French marigold) in acute L-NAME induced hypertensive rats

Abstract

The work is aimed to evaluate the blood pressure reducing effect of constituents from methanol extract and associated constituents of Tagetes patula flowers in normotensive and L-NAME induced hypertensive rats. The HPLC analysis of methanol extract of Tagetes patula flowers (JFM) resulted in the quantitative identification and percent comparison of four phenolic constituents, protocatechuic acid (PA), methyl protocatechuate (MPA), patulitrin (TRIN) and patuletin (PAT). All the extracts, fractions and compounds examined showed significant blood pressure lowering activity. Patulitrin (TRIN) which has emerged as the major constituent (15.33%) of T. patula flowers showed significant 30% and 68% fall in blood pressure in normotensive and L-NAME induced hypertensive rats respectively. The patuletin (PAT), which is an aglycone of TRIN displayed high percentage (84%) of antihypertensive activity. Further, comprehensive and advanced studies on these constituents may result in preparation of an effective blood pressure lowering medicine with active precious rare flavonoids, patuletin and patulitrin.

Graphical Abstract

Keywords:

• Tagetes patula
• methanol extract
• patulitrin
• patuletin
• % fall in MABP

Acknowledgments

The authors thankful to the Higher Education Commission, Islamabad, Pakistan for the financial support to Samina Bano through the ‘‘Indigenous 5000 Fellowship Program-Batch V’’.

Disclosure statement

No potential conflict of interest was reported by the authors.

Correction Statement

This article has been corrected with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This publication was supported by Late, Prof. Dr. Syed Iqbal Ahmad, former Dean, Faculty of Pharmacy, Hamdard University, Karachi, Pakistan. Regretfully he is no more with us. His precious guidance and enthusiastic support enabled us to carry out this research work. The author(s) reported there is no funding associated with the work featured in this article.