LEAD TOLERANCE AND ACCUMULATION IN PTERIS VITTATA AND PITYROGRAMMA CALOMELANOS, AND THEIR POTENTIAL FOR PHYTOREMEDIATION OF LEAD-CONTAMINATED SOIL

Abstract

A field survey was conducted to search for Pb accumulation in fern species at Bo Ngam Pb mine, Thailand. Eleven fern species including Pteris vittata accumulated Pb in the range of 23.3–295.6 mg kg⁻¹ in the aboveground parts. Hydroponic, pot, and field trial experiments were carried out to investigate Pb-accumulation ability in ferns; including P. vittata and the ornamental species, Pityrogramma calomelanos, Nephrolepis exaltata cv. Gracillimum, and N. exaltata cv. Smirha. In hydroponic experiment, Pi. calomelanos accumulated the highest concentration of Pb (root 14161.1 mg kg⁻¹, frond 402.7 mg kg⁻¹). The pot study showed that P. vittata, Pi. calomelanos, and N. exaltata cv. Gracillimum grew well when grown in soil Pb at 92900 mg kg⁻¹. N. exaltata cv. Gracillimum accumulated the highest Pb concentration in the frond (5074 mg kg⁻¹) and P. vittata accumulated the highest Pb concentration in the root (16257.5 mg kg⁻¹). All fern species exhibited TF values less than 1 in both hydroponic and pot experiments. When P. vittata and Pi. calomelanos were grown at mine soils for 6 months, P. vittata tolerated higher soil Pb (94584–101405 mg kg⁻¹) and accumulated more Pb in frond (4829.6 mg kg⁻¹) and showed TF > 1 after 2 months of growth. These results indicated that P. vittata can be potentially useful for phytoremediation of Pb-contaminated soil.

KEY WORDS:

• Pb phytoremediation
• Pteris vittata
• Pityrogramma calomenlanos
• field survey
• hydroponic study
• pot study
• field trial study

ACKNOWLEDGMENTS

This research work was supported by a grant from the Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program and the Post-Graduate Education, Training and Research Program in Environmental Science, Technology, and Management under Higher Education Development Project of the Commission on Higher Education, Ministry of Education, and Mahidol University, Bangkok, Thailand. We are grateful to Assistant Professor Philip Round and Mr. Laird Allen for assistance with the proof reading of the manuscript.

Notes

^aEC : electrical conductivity

^bOM : organic matter. Field survey : line transect direction; N = north, W = west, S = south, E = east.