EXPLORING THE PHYTOREMEDIATION POTENTIAL OF CACTUS (NOPALEA COCHENILLIFERA SALM. DYCK.) CELL CULTURES FOR TEXTILE DYE DEGRADATION

Abstract

Cactaceae Nopalea cochenillifera cell cultures and intact plants (cladodes) transform various toxic textile dyes, including Red HE7B into less phytotoxic, non-hazardous metabolites. The [tentative] pathway analysis demonstrates that Red HE7B is transformed into 3-amino-5-imino-5,8-dihydronaphthalen-2-ol, 2-amino-6-(carboxycarbonyl)-3,4-dihydroxybenzoic acid, 4-aminophenol, and phenol. The significant induction of the activities of intracellular laccase (687%), tyrosinase (219%), azoreductase (144%), and 2,6-dichlorophenol-indophenol reductase (167%) indicated the involvement of these enzymes in the transformation pathways of Red HE7B but these enzymes have not been definitively linked to the phytotransformation of this toxic dye. The present foundation work could add another plant candidate for phytoremediation of undesirable products from industry wastes and harmful chemicals.

KEY WORDS:

• cell cultures
• Nopalea cochenillifera
• phytoremediation
• tyrosinase
• Laccase
• Red HE7B

ACKNOWLEDGMENTS

VSA is thankful to Department of Biotechnology, Shivaji University, Kolhapur for Departmental Research Fellowship (DRF). VAB wishes to thank Council for Scientific and Industrial Research (CSIR), New Delhi for Emeritus Scientist Fellowship.