Examination of correlation between histidine and nickel absorption by Morus L., Robiniapseudoacacia L. and Populus nigra L. using HPLC-MS and ICP-MS

ABSTRACT

In this study, HPLC-MS and ICP-MS methods were used for the determination of histidine and nickel in Morus L., Robinia pseudoacacia L., and Populus nigra L. leaves taken from industrial areas including Gaziantep and Bursa cities. In the determination of histidine by HPLC-MS, all of the system parameters such as flow rate of mobile phase, fragmentor potential, injection volume and column temperature were optimized and found to be 0.2 mL min⁻¹, 70 V, 15 µL, and 20°C, respectively. Under the optimum conditions, histidine was extracted from plant sample by distilled water at 90°C for 30 min. Concentrations of histidine as mg kg⁻¹ were found to be between 2–9 for Morus L., 6–13 for Robinia pseudoacacia L., and 2–10 for Populus nigra L. Concentrations of nickel were in the ranges of 5–10 mg kg⁻¹ for Morus L., 3–10 mg kg⁻¹ for Robinia pseudoacacia L., and 0.6–4 mg kg⁻¹ for Populus nigra L. A significant linear correlation (r = 0.78) between histidine and Ni was observed for Populus nigra L., whereas insignificant linear correlation for Robinia pseudoacacia L. (r = 0.22) were seen. Limits of detection (LOD) and quantitation (LOQ) were found to be 0.025 mg Ni L⁻¹ and 0.075 mg Ni L⁻¹, respectively.

KEYWORDS:

• histidine
• nickel
• hyperaccumulator plants
• ICP-MS
• HPLC-MS

Funding

This study was financially supported by the Scientific Investigate Projects of Firat University, Turkey (Project Number: FF.11.19).