Phytoremediation affects microbial development on a limestone quarry

ABSTRACT

Phytoremediation was used to regenerate a limestone quarry area. Plant growth mixed medium added over the quarry surface, consisting of a mixture of pyrolusite byproducts, natural soil, sand, and rice husk. Three different plant species: pine, cypress, and broom were planted at 9 randomized plots in order to assess the effects of vegetation on the microbial development, which was measured for the following 3 years. Substrate samples were analyzed for organic carbon content (C_org), microbial biomass (C_mic), basal CO₂ respiration activity (BR), alkaline phosphatase (ALP), and acid phosphatase activities at each plant specie and year. Furthermore, the ratio C_mic/C_org, the metabolic quotient (qCO₂), and the C mineralization quotient (qM) were determined. The highest survival rates occurred for broom (93.52%), followed by cypress and pine (82.41%) at the final year, while the content of C_mic, BR, and ALP was increased significantly under plants (pine, cypress, and broom) compared with control. C_mic content and BR was plant dependent. Cypress sites had the highest values of C_mic (214.9 μgCg⁻¹) and BR (112.8 μgCO₂-Cg⁻¹d⁻¹) at the 3^rd year. The plant root environment clearly enhances and regulates the microbial community, in correspondence to the species used. Below ground enhanced activity could fulfill the scope of phytoremediation strategies.

KEYWORDS:

• forest plant establishment
• microbial activity
• pyrolusite byproducts