Transgenic tobacco plants overexpressing a cold-adaptive nitroreductase gene exhibited enhanced 2,4-dinitrotoluene detoxification rate at low temperature

Abstract

Plants encounter many environmental factors such as low and high temperatures during phytoremediation processes. In this study, our aim was to produce the transgenic tobacco plants by using a newly characterized bacterial nitroreductase, Ntr, which was active at a broad range temperature in order to detoxify 2,4-dinitrotoluene (2,4-DNT) at lower temperature. The presence of Ntr and its heterologous expression was verified in T1 transgenic plants and their growing ability were determined under toxic amount of 2,4-DNT (35 µM). Fresh weight and dry weight of transgenic plants were significantly higher than wild type (WT) under toxic 2,4-DNT at 22 °C, indicating higher growth capacity of the transgenics. Transgenic plants also showed a higher tolerance than WT when exposed to 2,4-DNT at 15 °C. Moreover, transformation rate of 2,4-DNT was gradually decreased through decreasing temperatures in WT media, however, it was increased through decreasing temperatures in transgenic plant TR3-25 media and it had the highest transformation rate (54%) of 2,4-DNT at 4 °C. Correlatively, 2,4-DNT treatment at 4 °C led to a significant decrease in H₂O₂ level in transgenic plants. Thus, transgenic plants overexpressing nitroreductase might have an important advantage for phytoremediation of toxic nitroaromatic compounds in field applications at low temperatures.

Keywords:

• 2,4-dinitrotoluene (2,4-DNT)
• cold
• Nicotiana tabacum
• nitroreductase (Ntr)
• phytoremediation
• Staphylococcus saprophyticus

Acknowledgement

This project was supported by The Scientific and Technological Research Council of Turkey (Project No: 214Z157).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Author contribution

Doğa Selin Kayıhan performed experiments, analyzed data and wrote the manuscript. Ceyhun Kayıhan helped performing experiments and writing the manuscript. Yelda Özden Çiftçi received research grant and managed the project and assisted on writing the manuscript.