Synthesis and Evaluation of Oligonucleotide-Conjugated Pyrrole Polyamide-2′-Deoxyguanosine Hybrids as Novel Gene Expression Control Compounds

Abstract

DNA oligonucleotide-conjugated pyrrole polyamide-2′-deoxyguanosine hybrids were synthesized and examined as novel gene expression control compounds. The T_m values and circular dichroism spectral analyses showed that the oligonucleotide-conjugated hybrids possess high DNA recognition and a very high binding affinity for DNA that includes the pyrrole polyamide binding sequence.

Keywords:

• Gene therapy agents
• pyrrole polyamide-2′-deoxyguanosine hybrids
• minor groove binder
• antisense oligonucleotides
• antigene drugs

Acknowledgments

This work was supported in part by a grant for private universities provided by the Promotion and Mutual Aid Corporation for Private Schools of Japan.