Is There Any Interaction Between Telomeric DNA Structures, G-Quadruplex and I-Motif, with Saffron Active Metabolites?

Abstract

Telomeric DNA contains some unique secondary structures, such as G-quadruplex and I-motif. These structures may be stabilized or changed by binding to specific proteins or small molecules. Herein, we report the in vitro effect of crocin, crocetin, picrocrocin, and safranal on these structures. Circular dichroism (CD) data indicate that crocetin has higher affinity for these structures. Safranal and crocin induce little change in the I-motif and G-quadruplex, respectively. The molecular docking confirms the experimental data and indicates the minor groove binding of ligands with G-quadruplex. The possibility for application of these ligands as sequence-specific drugs should be further investigated.

Keywords:

• Saffron
• carotenoids
• monoterpene aldehydes
• DNA secondary structure
• circular dichroism
• molecular docking