Two DNA binding modes of a zinc-metronidazole and biological evaluation as a potent anti-cancer agent

Abstract

A complex of metronidazole (MTZ) with zinc ion was synthesized and characterized by UV-Vis, Fourier transform infrared (FT-IR), ¹H-NMR, X-ray crystallography and thermal gravimetric-differential thermal analysis (TG-DTA). The cytotoxicity effect of the synthesized complex investigated over SKNMC, A549, MCF-7, and MCDK cell lines and the results have shown that it has high cytotoxic potential over cancer cell lines. In order to clarify the mechanism of cell cytotoxicity, the oxidative stress and binding of the complex to the calf thymus-DNA studied by evaluating the intrinsic binding constant and defining thermodynamic parameters of complex over the DNA accompanying with in silico molecular modeling method. For this purpose, the complex optimized at the B3LYP/LANL2DZ level and docked over the DNA structure. The results revealed that the metronidazole-zinc complex interacted with DNA via hydrogen binding and electrostatic interaction to the minor groove region and phosphate backbone of DNA, respectively.

KEYWORD:

• s: Zinc-metronidazole complex
• two modes DNA binding
• cytotoxic
• oxidative stress
• molecular docking

Acknowledgments

We gratefully acknowledge Vice Chancellor for Research and Technology, Kermanshah University of Medical Sciences for financial support. This work was performed in partial fulfillment of the requirements for a Pharm. D by Nosaibeh Shabrandi in the Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.