Species-dependent patterns of incorporation of purine mononucleotides and nucleosides by lactic acid bacteria

Abstract

Although most lactic acid bacteria do not directly incorporate purine nucleotides, the strain Lactobacillus gasseri PA-3 was found to incorporate purine mononucleotides. To determine whether the direct uptake of purine mononucleotides is dependent on the species or strain of lactic acid bacteria, incorporation of purine mononucleotides was assessed in L. gasseri, Lactcoccus lactis sbsp. lactis, Streptococcus thermophilus and other species of lactic acid bacteria. Each bacterial strain was incubated with ³²P-AMP or ¹⁴C-adenosine and the incorporation of each purine was evaluated by measuring their radioactivity. All investigated strains of L. gasseri incorporated ³²P-AMP, whereas strains of S. thermophilus and most strains of L. lactis did not. Incorporation of ³²P-AMP into strains of Pediococcus was dependent on the strain or species of that genus of bacteria. All investigated strains, except for one strain of L. gasseri, incorporated ¹⁴C-adenosine, with S. thermophilus, L. lactis and Pediococcus generally displaying greater incorporation of ¹⁴C-adenosine than L. gasseri. Although most lactic acid bacteria such as S. thermophiles and L. lactis do not incorporate purine mononucleotides, some species such as L. gasseri directly incorporate purine mononucleotides. These findings indicate that the preferential incorporation of purine mononucleotides or nucleosides by lactic acid bacteria is dependent on the species or strain.

Keywords:

• Lactic acid bacteria
• Lactobacillus gasseri
• purine mononucleotide
• purine nucleoside
• Lactococcus lactis sbsp. lactis
• Streptococcus thermophilus