Electrochemical Study on Behavior of EuMo₂ Complex and Its Interaction With DNA

Abstract

The electrochemical behavior of complex EuMo₂ (Mo = Morin, 2′,3,4′5,7-pentahydroxyflavone) and its interactions with calf thymus DNA were studied using cyclic voltammetry (CV) and double potential step chronocoulometry (DPSCC) at glass carbon electrode (GCE) and DNA modified GCE, respectively. Information such as diffusion coefficient (D), rate constant (k _s) of EuMo₂ and intrinsic binding constant (K), binding numbers (n) of bound species per DNA (bp) were obtained. EuMo₂ can bind to DNA, and the binding mode is intercalation. By nonlinear fitting with Langmuir equation, a K of 1.02 × 10⁶ M⁻¹ and an n of 1 were obtained.

Keyword:

• EuMo₂ complex
• Calf thymus DNA
• Intercalation mode
• DNA modified electrode

The present work was supported by a grant from the National Natural Science foundation of China (No. 20275031, 20335030) and KJCXGC-01 from Northwest Normal University