Deamination of 2′,3′-O-Isopropylideneadenosine-5′- Carboxylic Acid Catalyzed by Adenosine Deaminase (ADA) and Adenylate Deaminase (AMPDA): Influence of Substrate Ionization on the Activity of the Enzymes

Abstract

Adenosine deaminase (ADA) and adenylate deaminase (AMPDA) catalyze the deamination of 2 ′,3 ′-O-isopropylideneadenosine-5′-carboxylic acid to the corresponding inosine derivative and dependence of the rate of enzymatic reaction on the ionization degree of the substrate has been studied at different pH values.

Keywords:

• 2′,3′-O-isopropylideneadenosine-5′-carboxylic acid
• adenosine deaminase
• adenylate deaminase

This work has been financially supported by Università degli Studi di Milano (Fondi FIRST).

Notes

^a 20% conversion.

^b 46% conversion.