Triplex Formation Using Oligonucleotide Clamps Carrying 8-Aminopurines

Anna Aviñó Instituto de Biologia Molecular de Barcelona, C.S.I.C., Barcelona, Spain; Institute for Research in Biomedicine (IRB), Barcelona. Parc Científic de Barcelona, Barcelona, Spain

Marta G. Grimau Instituto de Biologia Molecular de Barcelona, C.S.I.C., Barcelona, Spain; Institute for Research in Biomedicine (IRB), Barcelona. Parc Científic de Barcelona, Barcelona, Spain

Margarita Alvira Instituto de Biologia Molecular de Barcelona, C.S.I.C., Barcelona, Spain; Institute for Research in Biomedicine (IRB), Barcelona. Parc Científic de Barcelona, Barcelona, Spain

Ramon Eritja Instituto de Biologia Molecular de Barcelona, C.S.I.C., Barcelona, Spain; Institute for Research in Biomedicine (IRB), Barcelona. Parc Científic de Barcelona, Barcelona, Spain

Raimundo Gargallo Facultat de Química, Universitat de Barcelona, Barcelona, Spain

Modesto Orozco Institute for Research in Biomedicine (IRB), Barcelona. Parc Científic de Barcelona, Barcelona, Spain; Facultat de Química, Universitat de Barcelona, Barcelona, Spain

Carlos González Instituto de Química Física Rocasolano, C.S.I.C., Madrid, Spain

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Abstract

The synthesis and properties of triplex-forming DNA clamps carrying 8-aminopurines are described. The stability of triple helices is enhanced by replacing purine bases with 8-aminopurine residues. These enhanced binding properties are used for the specific capture of polypyrimidine RNA/DNA sequences of interest.

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Acknowledgments

Project financed by E.E.C.C. STRP 014006 Nano3D, by Fundació La Caixa BM04-52-0 and by Spanish Ministry of Education BFU2005-23719-E, NAN2004-09415-C05-03 and BFU2004-02048.

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Triplex Formation Using Oligonucleotide Clamps Carrying 8-Aminopurines

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Triplex Formation Using Oligonucleotide Clamps Carrying 8-Aminopurines

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