Approach to the Synthesis of Natural and Modified Oligonucleotides by the Phosphotriester Method Using O-Nucleophilic Intramolecular Catalysis

Abstract

An approach to the solid phase synthesis of natural and modified oligonucleotides using phosphotriester technique has been developed. Particularly, this method allows the synthesis of ribo- and deoxyribo-oligonucleotides containing various 2 ′-modified mononucleotides as well as stereodefined nucleotide phosphorothioate analogues.

Keywords:

• Oligoribo- and deoxyribonucleotides
• stereospecific phosphotriester synthesis
• phosphorothioate analogues