Development of Artificial Restriction DNA Cutter Composed of Ce(Iv)/EDTA and PNA

Abstract

An artificial restriction enzyme, which we developed recently by combining Ce(IV)/EDTA and peptide nucleic acids, was used for PCR-free construction of a fusion protein. The fusion protein was successfully expressed in mammalian cells. This artificial DNA cutter can be also applied to site-selective scission of huge DNAs. Promising features of this novel tool were concretely evidenced.

Keywords:

• PNA
• cerium
• hydrolysis
• restriction enzyme
• DNA

This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, Culture and Technology, Japan.