Synthesis of Leishmania Cap-4 Intermediates, Cap-2 and Cap-3

Magdalena Lewdorowicz Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warsaw, Poland

Janusz Stepinski Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warsaw, Poland

Ryszard Kierzek Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland

Jacek Jemielity Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warsaw, Poland

Joanna Zuberek Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warsaw, Poland

Yael Yoffe Department of Life Sciences, Ben-Gurion University, Beer Sheva, Israel

Michal Shapira Department of Life Sciences, Ben-Gurion University, Beer Sheva, Israel

Ryszard Stolarski Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warsaw, Poland

Edward Darzynkiewicz Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warsaw, Poland

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Abstract

Synthesis of Leishmania mRNA 5′-cap analogs, m⁷Gpppm₂ ⁶AmpAm (cap-2), and m⁷Gpppm₂ ⁶AmpAmpCm (cap-3) is reported. Binding affinities of those cap analogs for LeishIF4E proteins were determined using fluorescence spectroscopy. Cap-3 showed similar affinity to LeishIF4Es compared to the mature trypanosomatids cap structure (cap-4).

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Supported by the Polish Ministry of Science and Higher Education, Grants No. 3 T09A 153 29 and 2 P04A 006 28 and Howard Hughes Medical Institute Grant (to E.D.) No. 55 005604.

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Synthesis of Leishmania Cap-4 Intermediates, Cap-2 and Cap-3

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