Labeling of Oligonucleotides with DTPA and DOTA on Solid Phase

Abstract

Oligonucleotide conjugates labeled with metal chelates of diethylenetriaminepentaacetic acid (DTPA) and tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) were synthesized on solid phase using appropriate nucleosidic phosphoramidite building blocks ( 3 , 4 ) and a modified deprotection-metal chelation protocol. The major differences on the properties of the oligonucleotide conjugates also are discussed.

Keywords:

• Oligonucleotide
• DTPA
• DOTA