RNA Cleavage by 2,9-Diamino-1,10-Phenanthroline PNA Conjugates

Abstract

We report on the synthesis of 2,9-diamino-1,10-phenanthroline PNA conjugates as well as on their action in cleavage of a target RNA. Synthesis of the PNA conjugates are performed on solid support and the phenanthroline derivative is conjugated either to the amino-end or to a centrally positioned diaminopropionic acid in the PNA via a urea linker. Cleavage of the target RNA is achieved and compared to cleavage with the corresponding 2,9-dimethyl-1,10-phenanthroline and glycine conjugates.

Keywords:

• PNA conjugate
• solid phase synthesis
• artificial nuclease
• 2,9-diamino-1,10-phenanthroline