Mass Spectrometry Based Methods for Analysis of Nucleosides as Antiviral Drugs and Potential Tumor Biomarkers

Abstract

The intracellular analysis of the phosphorylated metabolites of some anti-HIV nucleosides by liquid chromatography or capillary electrophoresis coupled with tandem mass spectrometry (LC-MS/MS or CE-MS/MS) has been realized on human peripheral blood mononuclear cells (PBMC), with limit of quantitation (LOQ) that allow them to be quantitated intracellularly. We described also the analysis of modified urinary nucleosides as potential tumor biomarkers.

Keywords:

• Antiviral
• HIV
• nucleosides
• LC-MS/MS
• therapeutic drug monitoring

These studies were supported in part by a grant from Agence Nationale de Recherches sur le SIDA, the SIDACTION and Bristol-Myers Squibb.