2′,4′-BNA^NC: A Novel Bridged Nucleic Acid Analogue with Excellent Hybridizing and Nuclease Resistance Profiles

Abstract

Oligonucleotides modified with 2 ′,4 ′-BNA ^NC (N-H)/(N-Me) monomers exhibited excellent hybridizing and nuclease resistance properties. Duplex and triplex thermal stabilities were greatly enhanced by incorporating 2′,4′-BNA ^NC (N-H) and (N-Me) monomers and nuclease resistance was tremendously higher than that of natural oligonucleotide.

Keywords:

• Nuclease resistance
• stability
• BNA
• oligonucleotide

S.M.A.R. is on leave from the Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh.

Notes

^a Conditions: See Materials and Methods.

^b Δ T _m/mod. = changes in melting temperature per single modification.

^a Conditions: See Materials and Methods.