Nucleoside Phosphorylases from Clostridium Perfringens in the Synthesis of 2′,3′-Dideoxyinosine

Abstract

Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2′,3′-dideoxyinosine (ddI). In one-pot synthesis using Clostridium phosphorylases, 2′,3′-dideoxyuridine and hypoxanthine were converted to ddI at yield of about 30%.

Keywords:

• Nucleoside phosphorylase
• Clostridium perfringens
• biocatalysis
• 2′,3′-dideoxyinosine
• anti-viral drugs