PARP Activity and NAD Concentration in PMC from Patients Affected by Systemic Sclerosis and Lupus Erythematosus

B. Cerboni Department of Molecular Biology, Section of Biological Chemistry, University of Siena, Siena, Italy

A. Di Stefano Department of Molecular Biology, Section of Biological Chemistry, University of Siena, Siena, Italy

V. Micheli Department of Molecular Biology, Section of Biological Chemistry, University of Siena, Siena, Italy

G. Morozzi Clinical Medicine and Immunological Sciences Department-Division of Rheumatology, University of Siena, Italy

G. Pompucci Department of Molecular Biology, Section of Biological Chemistry, University of Siena, Siena, Italy

S. Sestini Department of Molecular Biology, Section of Biological Chemistry, University of Siena, Siena, Italy

Abstract

The enzyme poly(ADP-ribose) polymerase (PARP-1, EC 2.4.2.30) is activated by DNA strand breaks caused by several agents and utilizes NAD to form polyADPR, bound to acceptor proteins. The involvement of PARP-1 in autoimmune diseases has been suggested: antiPARP autoantibodies are described in systemic lupus erythematosus (SLE), DNA strand breaks have been evidenced in systemic sclerosis (SSc). We tested poly(ADP-ribosyl)ation activity and NAD concentration in PMC from patients affected by SLE or SSc and from controls. Lower PARP-1 activity and higher NAD concentration were observed in pathological conditions than controls, supporting the role of PARP-1 activation in modulating NAD concentration.

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PARP Activity and NAD Concentration in PMC from Patients Affected by Systemic Sclerosis and Lupus Erythematosus

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PARP Activity and NAD Concentration in PMC from Patients Affected by Systemic Sclerosis and Lupus Erythematosus

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