Properties of DNA Duplexes Containing an Abasic Site: Effect of Nucleotide Residue of the Opposite Part of a 2-Deoxyribosylformamide Residue

Abstract

To elucidate the conformational properties of a DNA duplex with a 2-deoxyribosylformamide residue (dF), oligomers containing this abasic residue and a propanediol residue in the center of the helix were synthesized and characterized by UV absorption and CD. By a comparison of the Tms of the duplexes containing a dF residue and a propanediol residue (P), the stability of the duplex containing the abasic damage was judged to depend on the kind of nucleotide residue on the opposite side of the abasic residue rather than the kind of abasic residue.