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Abstract
Properties of a hammerhead-type RNA enzyme system that consists of three RNA oligomers are described. The substrate 11-mer was catalytically cleaved in the presence of the enzyme components, 12-mer and 16-mer, and Mg2+ ions. Effects of various metal ions, Mg2+ concentration, and temperature on the cleavage reaction of the substrate were examined. We obtained evidence suggesting that religation of the cleaved products actually occurs. From CD titration experiments with MgCl2 using non-cleavable substrates, binding constants of Mg2+ to the complexes were estimated.