Molecular Mechanism of 5-Fluoro-2′-deoxyuridine-induced dNTP Imbalance Cell Death: Purification of an Endonuclease Involved in DNA Double Strand Breaks During dNTP Imbalance Death

Hwa-shin Hwang Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700

Masashi Ohtani Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700

Tohru Nakazawa Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700

Tatsushi Igaki Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700

Osamu Masui Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700

Shiro Kankawa Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700

Chikao Nakayama Discovery Research Laboratories II, Sumitomo Pharmaceuticals Research Center, Kasugade, Osaka, 554, Japan

Sei Yoshida Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700

Akiko Yoshioka-Hiramoto Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700

Yusuke Wataya Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700

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Abstract

We have detected, isolated and purified an endonuclease from 5-fluoro-2′-deoxyuridine-treated FM3A cells. The molecular mass of the endonuclease was approximately 40 kDa as judged by sodium dodecyl sulfate-polyacrylamide DNA-containing gel electrophoresis. The endonuclease causes double strand breaks in DNA, with an optimum pH at 6.

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Molecular Mechanism of 5-Fluoro-2′-deoxyuridine-induced dNTP Imbalance Cell Death: Purification of an Endonuclease Involved in DNA Double Strand Breaks During dNTP Imbalance Death

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Molecular Mechanism of 5-Fluoro-2′-deoxyuridine-induced dNTP Imbalance Cell Death: Purification of an Endonuclease Involved in DNA Double Strand Breaks During dNTP Imbalance Death

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