A General Approach for the Hydroxy Group Functionalization of Synthetic Oligonucleotides

Abstract

Generally applicable non-nucleosidic solid supports and phosphoramidite building blocks that enable attachment of various tethers to the hydroxy groups of oligonucleotides were prepared. The key feature of the structure of these reagents is an ester bond of moderate reactivity which allows postsynthetic introduction of tethers by reaction with substituted alkylamines.