Chemical Synthesis of RNA-Fragment Analogues That Have Phosphorothioate Linkages of Identical Configuration

Abstract

Oligouridine all-Rp-phosphorothioates were synthesized using the H-phosphonate approach followed by sulfurisation with S₈ and treatment with nuclease P1. The degree of stereoselection in the couplings is reported for different 2′-O-silyl protecting groups. The influence of the heterocyclic base was also investigated.