Synthesis and Characterization of Short Oligonucleotide Segments Containing Nonnatural Internucleoside Amine- and Amide Linkages

Abstract

The synthesis of thymidine dimers in which the phosphodiester linkage has been replaced by either piperazine or N,N'-dimethylethylenediamine are described. The dimers containing piperazine were incorporated into oligodeoxynucleotides on which thermal and enzymatic stability experiments were performed.