Preparation of Oligodeoxynucleotides Containing 6-Methylthiopurine Residues by Chemical Synthesis or Specific Methylation

Yao-Zhong Xu Cancer Research Campaign Nitrosamine-Induced Cancer Research Group Department of Biochemistry and Molecular Biology, University College London Gower Street, London, WC1E 6BT, England

Qinguo Zheng Cancer Research Campaign Nitrosamine-Induced Cancer Research Group Department of Biochemistry and Molecular Biology, University College London Gower Street, London, WC1E 6BT, England

Peter F. Swann Cancer Research Campaign Nitrosamine-Induced Cancer Research Group Department of Biochemistry and Molecular Biology, University College London Gower Street, London, WC1E 6BT, England

Abstract

Two methods (chemical synthesis and specific methylation) are described for the preparation of oligodeoxynucleotides containing 6-methylthiopurine residues. 6-Methylthiopurine phosphoramidite (6) is prepared and incorporated into oligomers. Methylation with methyl iodide of 6-thiopurine (or 6-thioguanine) in oligonucleotides also leads to exclusive production of 6-methylthiopurine (or 6-methylthioguanine) oligomers.

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Preparation of Oligodeoxynucleotides Containing 6-Methylthiopurine Residues by Chemical Synthesis or Specific Methylation

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Preparation of Oligodeoxynucleotides Containing 6-Methylthiopurine Residues by Chemical Synthesis or Specific Methylation

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