Hybridization Properties of the 5-Methyl-isocytidine/isoguanosine Base Pair in Synthetic Oligodeoxynucleotides

Thomas Horn Nucleic Acids Systems, Chiron Corp., Emeryville, CA, 94608, USA

Chu-An Chang Nucleic Acids Systems, Chiron Corp., Emeryville, CA, 94608, USA

Mark L. Collins Nucleic Acids Systems, Chiron Corp., Emeryville, CA, 94608, USA

Abstract

The hybridization properties of the 5-methyl-isocytidine/isoguanosine base pair in synthetic oligodeoxynucleotides have been investigated. The 5-methyl-isocytidine/isoguanosine base pair has been determined to be isoenergetic with the cytidine/guanosine, and each base can effectively discriminate mismatches.

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Hybridization Properties of the 5-Methyl-isocytidine/isoguanosine Base Pair in Synthetic Oligodeoxynucleotides

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Hybridization Properties of the 5-Methyl-isocytidine/isoguanosine Base Pair in Synthetic Oligodeoxynucleotides

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