Synthesis and Antibody Mediated Detection of 2,4-Dinitrophenyl (DNP) Labelled Oligonucleotides

A. Davison Cruachem Ltd., West of Scotland Science Park, Glasgow, U. K.

G. Duckworth Cruachem Ltd., West of Scotland Science Park, Glasgow, U. K.

M. V. Rao Cruachem Ltd., West of Scotland Science Park, Glasgow, U. K.

J. McClean Department of Chemistry, University of Edinburgh, Edinburgh, U. K.

J. Grzybowski Department of Chemistry, University of Edinburgh, Edinburgh, U. K.

P. Potier Department of Chemistry, University of Edinburgh, Edinburgh, U. K.

T. Brown Department of Chemistry, University of Edinburgh, Edinburgh, U. K.

H. Cubie Regional Virus Laboratory, City Hospital, Greenbank Drive, Edinburgh, U. K.

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Abstract

Different DNP phosphoramidites based on non-nucleoside and nucleoside backbone molecules are developed and used in the multiple labelling of oligonucleotides during the solid phase synthesis. It is demonstrated that the antibody mediated detection of DNP labelled oligonucleotides is comparable to that of digoxigenin, biotin and fluorescein.

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Synthesis and Antibody Mediated Detection of 2,4-Dinitrophenyl (DNP) Labelled Oligonucleotides

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Synthesis and Antibody Mediated Detection of 2,4-Dinitrophenyl (DNP) Labelled Oligonucleotides

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