Properties and Anti-HIV Activity of Circular Sense and Antisense Oligonucleotides

H. Yamakawa Rational Drug Design Laboratories, Misato, Matsukawa, Fukushima, Fukushima, 960-12, Japan

T. Ishibashi Department of Industrial Chemistry, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba, 275, Japan

H. Nakashima Department of Microbiology, Yamanashi Medical University, Nakakoma-gun, Yamanashi, 409-38, Japan

N. Yamamoto Department of Microbiology, Tokyo Medical and Dental University School of Medicine, Yushima, Bunkyo-ku, Tokyo, 113, Japan

K. Takai Department of Industrial Chemistry, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba, 275, Japan

H. Takaku Department of Industrial Chemistry, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba, 275, Japan

Abstract

The circularization of a 48 mer oligonucleotide was investigated by using enzymatic ligation with T4 DNA ligase. This method yielded 30% circular molecules. The circular oligonucleotides are molecules with no free ends and are therefore more resistant to exonuclease attack. The circular oligonucleotide has remarkably increased Tm values as compared to the nicked and double stranded DNAs. However, the RNase H activity is lower than that with a single stranded DNA. We also describe the anti-HIV activity of a circular oligonucleotide.

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Properties and Anti-HIV Activity of Circular Sense and Antisense Oligonucleotides

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Properties and Anti-HIV Activity of Circular Sense and Antisense Oligonucleotides

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