Age-related Changes in Oxidative DNA Damage and Benzo(a)pyrene Diolepoxide-I (BPDE-I)-DNA Adduct Levels in Human Stomach

Abstract

This study was conducted in order to obtain information on the association between age and oxidative DNA damage and benzo[a]pyrene diolepoxide-I (BPDE-I)–DNA adduct levels in the stomach tissues of normal subjects (n = 113). Subjects ranged from 17 to 75 yr. The levels of 8-hydroxy-2’-deoxyguanosine (8-OHdG), a biomarker of oxidative DNA damage, were measured by high-performance liquid chromatography–electrochemical detector (HPLC-ECD). BPDE-I–DNA adduct levels were quantitatively measured by enzyme-linked immunosorbent assay (ELISA) using the monoclonal antibody 8E11. Increase in 8-OHdG levels was observed in subjects from 31 to 60 yr of age. Higher levels of 8-OHdG were observed in the stomach tissues of those in the 51–60 yr old group. Although Helicobacter. Pylori-positive group subjects had higher 8-OHdG levels than those in the H. pylori-negative group, no age-related changes in 8-OHdG levels were observed in these groups. The levels of BPDE-I–DNA adduct also markedly increased with age. Higher levels of BPDE-I–DNA adduct were observed in subjects aged 61–70 yr, but this difference was not significant from other age groups. These results provide evidence that there is a progressive age-dependent accumulation of oxidative DNA damage and of BPDE-I–DNA adducts in human stomach tissues. It is possible that such damage contributes to the known increased incidence of gastric tumor with aging.