Screening of Differential Expression Genes in Bone Marrow Cells of Radon-Exposed Mice

Abstract

This study was designed to screen for differential expression genes in bone marrow cells of mice exposed to radon inhalation. Based upon established pathological findings in mouse, differential screening of gene expressions was conducted by using the SSH method. Among 285 cDNA clones selected from both forward- and reverse subtracted libraries, 45 were chosen for their differential expressions based on reverse Northern blot and quantitative real-time PCR analysis. Of these, up-regulation of the mRNA levels of E-cadherin and down-regulation of the replication protein A1 (RPA1) and casein kinase 1 delta (CKI delta) were also verified by a quantitative real-time PCR. Biological roles of these obtained cDNAs are described and the results of the screening may provide important clues for further investigations of the adverse molecular events induced by radon exposure.

Keywords:

• Radon
• mouse
• Differential gene expression
• Suppression subtractive hybridization (SSH)

ACKNOWLEDGEMENTS

This study was supported by National Science Foundation of China (30371226, 30671784), Key Basic Research Foundation of Jiangsu Province (05KJA33013) and Doctoral Research Foundation of China Higher Education (20050285009).