Mikania glomerata Sprengel (Asteraceae) Influences the Mutagenicity Induced by Doxorubicin without Altering Liver Lipid Peroxidation or Antioxidant Levels

Abstract

As shown in numerous studies, natural compounds may exert adverse effects, mainly when associated with some drugs. The hydroalcoholic extract of Mikania glomerata is the pharmaceutical form present in commercially available syrup used for the treatment of respiratory diseases in popular Brazilian medicine. The objective of the present investigation was (1) to evaluate the preventive effects of standardized hydroalcoholic extract of M. glomerata (MEx) against antitumoral drug doxorubicin (DXR)-induced micronucleated polychromatic erythrocytes (MNPCE) in a subchronic assay in mice, and (2) to determine the liver content of malondialdehyde (MDA) and the antioxidants glutathione (GSH) and vitamin E (VE). Male Swiss mice were treated for 30 d with MEx added to drinking water, combined or not with DXR (90 mg/kg body weight) injected intraperitoneally (ip) 24 h before analysis. The results demonstrated that MEx produced no genotoxic damage, but significantly increased the frequency of MNPCE induced by DXR, indicating a drug–drug interaction. This rise was not accompanied by lipid peroxidation or antioxidants level reduction, as measured by MDA, GSH, and VE. Despite the presence of coumarin (a known antioxidant), MEx may exert adverse effects probably in association with mutagenic compounds, although this effect on DNA damage did not involve oxidative stress.

Acknowledgments

The authors are grateful to Fundação Herbarium de Saúde e Pesquisa, Paraná, Brazil, and University of Franca, São Paulo, Brazil, for contributing to the execution of this project. They are also indebted to Dr. Denise Crispim Tavares for allowing us to perform this research at her laboratory. The authors are also grateful to Ana Paula Carolina Bueno for running the GC analysis.