12-hydroxy oleic acid impairs endothelium-dependent vasorelaxation

ABSTRACT

Diesel and biodiesel emissions exposures reduce vascular responsiveness in vivo, but the components of PM responsible for this effect are poorly understood. Fatty acids (FAs) represent a significant fraction of the compounds that make up organic combustion by-products, and may be involved in vascular responses following inhalation. It was hypothesized that vascular tissue exposed to a model FA might impair responses to vasoactive agonists ex vivo. Rat aortic rings were exposed to oleic acid or 12-hydroxy oleic acid and responses determined by myography. 12-Hydroxy oleic acid was found to significantly reduce endothelium-dependent vasodilation at sub-cytotoxic concentrations. This approach demonstrates the potential for FAs, especially oxidized forms, to play a role in the vascular responses observed following air pollution exposure.

KEYWORDS:

• Fatty acids
• endothelium
• vascular response
• air pollution

Disclaimer

This article has been cleared by the US EPA and does not represent official EPA policy. Mention of trade names or corporations does not constitute a recommendation.

 

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Additional information

Funding

This work was supported by Department Of Energy and US Environmental Protection Agency Interagency Agreement 92429801; and US EPA Grant CR-83578501 to The University of North Carolina at Chapel Hill.