Evaluation of DNA and chromosomal damage in two human HaCaT and L02 cells treated with varying triclosan concentrations

ABSTRACT

Triclosan has been used in a large number of consumer products and concerns have been raised over regarding potential genotoxicity. However, the genotoxicity of triclosan has not been assessed in normal human cells. The aim of this study was to examine the potential genotoxicity using the comet assay and micronucleus (MN) test to detect DNA damage and chromosomal breakage attributed to triclosan in human keratinocyte HaCaT and hepatic L02 cells. The concentrations of triclosan selected for the comet assay and MN test were based upon preliminary results from cytotoxicity testing in order to reduce cytotoxic effects. The mutagenicity of triclosan was assessed in Salmonella reverse mutation assay (Ames test). Results of comet assay showed that 5, 7.5 or 10 μM triclosan did not markedly affect olive tail moment (OTM) in HaCaT and L02 cells. In addition, no significant alterations in MN frequency were found in cells treated with triclosan. Further, treatment with 10 μg/plate triclosan produced inhibitory effects in bacterium using Ames test, while 1 and 0.1 μg/plate triclosan did not markedly affect the number of colonies or mutant frequencies of Salmonella strains. Taken together, triclosan did not cause DNA and chromosomal damage in HaCaT and L02 cells and did not induce gene mutations in Salmonella strains under our experimental conditions.

KEYWORDS:

• Triclosan
• genotoxicity
• cytotoxicity
• human cells
• risk assessment

Acknowledgments

We would like to thank Yun Ran for the technical assistance in Ames test with this project.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the grant from the National Science Foundation of China (No. 81773380) to Zunzhen Zhang.