Subacute inhalation toxicity testing with iodoform vapor

Abstract

Subacute inhalation experiments were conducted to determine the LC50 value for adult Sprague‐Dawley rats exposed to iodoform vapor. Each dose consisted of 5 males and 5 females that were together fora 7‐h exposure or sham exposure and then separated for observation over the subsequent 24‐h period. The rats were deprived of food and water during actual exposure or sham exposure. Exposures were conducted in a custom‐designed 75–1 glass chamber. Vapor concentrations were verified in samples taken from the exposure chamber. Under the conditions of the experiments the 7‐h LC50 was found to be 183 ppm. The second objective of these experiments was to determine the toxic effects of iodoform vapor on rats exposed for 7 h/d for 7 consecutive days. Three groups of 5 young adult male and 5 female rats were used. One group served as a sham control and the other groups were exposed to 1 and 14 ppm iodoform vapor. No significant differences were noted in food and water intake, urine and feces output, and intestinal transit performance in either exposed group. No remarkable changes were noted in SMA 12/60 blood values for either exposed group. The only histopathological manifestation noted was the presence of mineralized deposits in the medullary renal tubules of some of the rats from the 14‐ppm group.