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Original Articles

Measurement of Immunoglobulins G, A, and M Levels in B‐Lymphocytes Culture

, , , , &
Pages 303-311 | Received 16 May 2005, Accepted 21 Jun 2005, Published online: 06 Feb 2007
 

Abstract

Nephelometric immunoassays were developed for human IgG, IgA, and IgM quantitation in B‐lymphocytes culture media. They allowed measurement of immunoglobulin (Ig) levels over a broad range of concentrations with good accuracy and precision. The kinetics of Ig production in B‐lymphocyte cultures was followed and the mean amount of each Ig was determined in six different samples after three days of culture. The nephelometric immunoassays reported here could be used to study, in vitro, the influence of various molecules (inhibitory or amplifying effect) on B‐lymphocytes' functional capacities.

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