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Abstract
To prevent leaf senescence of petunia, the cytokinin biosynthetic gene isopentenyl transferase (ipt) was placed under the control of 35S promoter and introduced into petunia. PCR analysis showed an expected 0.5 Kb fragment of ipt gene in transgenic petunia. RT-PCR analysis indicated the expression of ipt gene in the transgenic lines. Leaves from transgenic plants remained green and healthy in normal culture condition, while the non-transformed plants turned to yellow. Transgenic plants showed a reduction in height and smaller leaf sizes. In transgenic lines, the internodes were shorter, and the roots grew slower than the non-transformed plants.
ACKNOWLEDGMENTS
The complete nucleotide sequences of GmIPT had been deposited in the NCBI nucleotide sequence database (Accession number: AY550884). This study was supported by the Natural Sciences Foundation (NSF) project of China and the Nature Science Foundation of Beijing City (5042016). The authors wish to thank Dr. Huiying Lv for providing seeds of soybean and the plasmid pBI121.