Relationship Between SDF-1G801A Polymorphism and its Expression in Tunisian Patients with Colorectal Cancer

Abstract

The aim was to evaluate the relationship between SDF-1G801A polymorphism and its immunohistochemical expression in colorectal cancer tissues in the Tunisian cohort. The molecular and immunohistochemical analysis showed that SDF-1G801A polymorphic variant was higher in CRC patients with TNM stage II and III, the SDF-1 expression was significantly increased from normal mucosa to primary tumor (p < 0.05). CRC patients have higher frequency of A allele (52.01%) than controls (26.8%) (P = 0.0001). Thus, SDF-1 polymorphism is a risk factor of colorectal cancer susceptibility in our population, the polymorph genotype of SDF-1 maybe associated with clinical manifestations in CRC patients in Tunisia.

Keywords:

• colorectal cancer
• SDF-1 SNP (single nucleotide polymorphism)
• SDF-1 expression
• PCR-RFLP
• immunohistochemestry

ACKNOWLEDGMENT

We thank the entire staff of the Department of Pathology and Cytology for their technical and secretarial assistance. We also would like to thank the medical staff of Mongi Slim Hospital of Tunis for their collaboration and excellent support.