The proportion–ratio on dipeptidyl aminopeptidase-4 (DP-4) inhibition by gelatin compared to synthetic sitagliptin

ABSTRACT

The current study aims to determine the inhibition activity gelatin against dipeptidyl aminopeptidase 4 (DP-4). Two commercial gelatins, i.e., bovine and fish skin gelatin and one extracted (in our laboratory) gelatin, i.e., fish bone gelatin were selected for analysis. Each gelatin have same protein pattern (75–245 kDa) on sodium dodecyl sulfate polyacrylamide gel electrophoresis with mean of protein concentration of 1.72 mg/mL. The inhibition activity was measured on the capacity to inhibit DP-4 by using Gly-Pro-p-nitroanilide as their substrate. The sitagliptin was used as standard comparison. Based on the percent inhibition, gelatin has been shown to be the prospective DP-4 inhibitor.

KEYWORDS:

• Dipeptidyl peptidase IV (DPP-4)
• gelatin
• incretin
• inhibitory activity
• diabetes mellitus type 2

Acknowledgments

This research was supported by Grant of Directorate General of Research and Development in Penelitian Kerjasama Antar Perguruan Tinggi (PKPT) scheme, Ministry Research Technology and Higher Education of Indonesia

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This work was supported by the Ministry of Research Technology and Higher Education of Indonesia [grant number: 037/KM/PNT/2018].